Ziege anti-Maus IgG2a Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN1118480

Hersteller Produkt- Nr.: 610-105-041

Kurzübersicht für Ziege anti-Maus IgG2a Antikörper (Alkaline Phosphatase (AP)) (ABIN1118480)

Target: IgG2a

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-105-041

Hersteller: Rockland

Verwendungszweck: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated Pre-adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Produktmerkmale: Anti-Mouse IgG1 Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).

Aufreinigung: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Mouse IgG2a heavy chain

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG2a Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, Immunohistochemistry, western blotting, as well as other anti IgG2a antibody based assays. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store secondary antibody at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgG2a

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.